Summary
Researchers identified that the aqueous extract of Triphala has significant antioxidant and anti-hemolytic activities, especially in G6PD-deficient red blood cells (RBCs). G6PD deficiency compromises antioxidant defense, making cells more susceptible to oxidative stress. The study utilized LC-MS and GC-MS analyses to characterize the phytochemical profile of the extract, identifying gallic acid as the main phenolic compound. Antioxidant activity tests showed that the extract reduced H2O2-induced hemolysis in both normal and deficient RBCs, without exhibiting cytotoxicity. Network pharmacology revealed that G6PD-related antioxidant regulation and inflammatory signaling pathways are key biological processes. Additionally, molecular docking analysis indicated that quinurenic acid binds strongly to proteins such as PTGS2 (COX-2) and ACE, suggesting significant modulatory potential. These findings highlight Triphala as a promising natural strategy to reduce oxidative damage in conditions related to G6PD deficiency.
Key points
- The Triphala extract demonstrated strong antioxidant activity, reducing H2O2-induced hemolysis by 30% in G6PD-deficient RBCs.
- Gallic acid was identified as the main phenolic compound, contributing to the antioxidant activity of the extract.
- Network pharmacology highlighted PPARG, PTGS2, and ACE as central proteins in oxidative stress and inflammation processes.
- Quinurenic acid exhibited a binding affinity below -7.0 kcal/mol with PTGS2 and ACE, suggesting stable interactions.
- Molecular dynamics simulations confirmed the stability of the quinurenic acid binding, with RMSD values close to 2.4 Å.
✦ For you
If you have concerns about the health of your red blood cells, consider including antioxidant-rich foods in your diet, such as colorful fruits and vegetables. Additionally, pay attention to how your body reacts to different foods and stressors. If you notice persistent symptoms, it is important to seek a qualified healthcare professional.
For practitionersExpand
Consider including Triphala (Triphala) in protocols for patients with G6PD deficiency, especially those showing signs of oxidative stress. Assess patient responses to the extract and adjust the formulation as necessary, taking into account the antioxidant activity and safety of the treatment.